Why Gleukos?

The high complexity of the human gut microbiota and the lack of know-how on all the individual players is a key challenge in gut microbiome research. Attempts have been made to define the microorganisms that constitute a healthy human gut microbiota but the scientific community has not yet reached a consensus to define the human beneficial intestinal microbial fingerprint. However, a number of observational studies have repeatedly shown a correlation between some bacterial populations and different physiological states, including those having an influence on human health.

Microbiology relies largely upon isolation and cultivation of microorganisms in the laboratory. However, the majority of the intestinal microorganisms cannot be cultivated; we know about their existence and identity mainly because the advent and refinement of novel sequencing methodologies during the last decade. Thanks to these techniques, we are able to extract the information encrypted in the microbiome, and subsequent bioinformatic-based analyses allow us to depict the diversity and potential metabolism of this microbial community. However, even using advanced massive sequencing methods, the physiology and metabolic capabilities of the majority of the microbial representatives of human gut have not been studied yet.

Project statistics

{{statistics.glycosides}} Glycosides

{{statistics.pathways}} Metabolic pathways

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Bioinformatics approach

This new in silico approach enables the systematic and large-scale study of glycolytic metabolic activities in the publicly available genomes of beneficial intestinal bacteria. The main aims are to predict and characterise the glycolytic capacities based on sequence similarity analysis and metabolic pathway annotations. Well-known public databases are at the core of the retrieval of functional enzyme and pathway annotations. Blastp 2.6.0 tool supports the search of glycoside hydrolase sequences in the collected organisms. KEGG Maps is used to generate the glycoside hydrolase functional maps, highlighting the pathways containing these activities and the encoding genes.