# Associate Mascot identifications from a HTM Mascot report file

This operation allows you to associate Mascot identifications from a HTM Mascot report file with a Maldi plate.

This operation has been designed to effectively manage experiments involving several Maldi plates and different sets of Mascot identifications. This way, instead of loading them separately and associating them in the momment of analyzing Spots data in the Integrated analysis view, users can associate all Maldi plates and Mascot identifications once, save them as a CSV file and then reusing this associated set of Spot Mascot identifications.

Usage

You can execute this operation by clicking the menu option Operations/Associate Mascot identifications from a HTM Mascot report file.

A dialog will appear allowing you to select the source HTM Mascot report file and MPL Maldi plate file. As you can see in the following image, this dialog requires you to provide the following information:

- Protein identifications: a HTM file containing the Mascot report.
- Minimum Mascot Score: the minimum Mascot Score for identifications to be loaded. Entries within a lower Mascot Score are not loaded.
- Remove duplicates: whether duplicated identifications must be skipped. Check this option to remove duplicated identifications.
- Maldi plate: A MPL file containing the Maldi plate.
- Spot identifications: optionally, a previously loaded Spot identifications to add the new data.

Associate Mascot identifications dialog

After clicking the Ok button, the associated identifications are added as a Spot Mascot identifications datatype to the clipboard and automatically opened in the Spot Mascot identifications table.